Entities are often raised to salience in conversational contexts even without being mentioned. For instance, a speaker might point to a bottle on the table and simply say `Water'. In most such cases, the speaker succeeds in communicating a determinate proposition; in this case that the bottle contains water. 63 I owe the point that Yablo's theory is unable to account for contextually determined intuitions of falsity in regards to 13 ; to Anne Bezuidenhout, who mentioned this in e-mail correspondence. I think Bezuidenhout's point is entirely correct, whereas she remained skeptical about the conclusion. Admittedly, I fail to understand why. Jill has been volunteering with the California AIDS Hotline since November of 1995. In addition to this, Jill has also volunteered for the AIDS LifeCycle and our annual AIDS Walk SF. Jill's dedication and commitment to our cause has been exemplary since day one and we are very glad and lucky to have her on our team of volunteers! Plus it only requires peanuts Planters ; in order to keep her coming back every week. Rotated around the pivot point until all three points are in line, ensuring that once the biopsy needle is passed through the trajectory guide the target will be encountered. Depending on the imaging characteristics of the target, the alignment stem can be filled either with saline or a saline and contrast mixture that allows it to be seen during the biopsy. After the alignment stem is inserted into the guide tube, it is rotated freely in space until all three points are aligned. This action generally takes two to five minutes while using ioMRI updates in near-realtime. Imaging along the entire length of the alignment stem in two different planes, depending where the target is located, verifies visually that the brain biopsy path is safe and accurate. Once the alignment has been established, the guide tube is secured in place and the biopsy needle is passed in a gradual step-wise fashion toward the target through the brain see Figure 2 ; . While the biopsy sample is being reviewed by the pathologist to confirm that diagnostic tissue is present, a series of three axial scans is performed to exclude the presence of intra-operative hemorrhage that may result from the biopsy. Since these scans are obtained before the conversion of intra-cellular.
Respondent: John Tedesco, Virginia Tech "The Sightbite: The Role of Visual Images in Political Communication." Daniel Schill, University of Kansas "An Argument for Multileveled Televised Political Debate Research." Elisia Cohen, St Louis University "The Presidential Candidates Debate: Viewer's Reactions to the Bush Kerry Debate." Clark Callahan, University of South Dakota; Terry Robertson, University of South Dakota "Political Engagement through Debates: Young Citizens' Reactions to the 2004 Presidential Debates." Mitchell S. McKinney, National Communication Association; Sumana Chattopadhyay, Marquette University 40747 2: 00 to Convention Center River Level Room 008 A and cilium. Abstract: We study the link between neutrino oscillations and leptogenesis in the minimal framework assuming an SO 10 ; see-saw mechanism with 3 families. Dirac neutrino masses being fixed, the solar and atmospheric data then generically induce a large masshierarchy and a small mixing between the lightest right-handed neutrinos, which fails to produce sufficient lepton asymmetry by 5 orders of magnitudes at least. This failure can be attenuated for a very specific value of the mixing sin 2 2e3 0.1, which interestingly lies at the boundary of the CHOOZ exclusion region, but will be accessible to future long baseline experiments. Keywords: Baryogenesis, Solar and Atmospheric Neutrinos, Cosmology of Theories beyond the SM. The rate of mature IgE transcription was determined by nuclear run-on analysis as described in detail previously 28, 42 ; . Briefly, 5 106 B cells were collected on ice at day 5 following activation and washed twice with cold PBS before resuspension in 5 ml cell lysis buffer containing 10 mM Tris-HCl pH 7.4 ; , 10 mM NaCl, 3 mM MgCl2, and 0.5% Nonidet P-40 Sigma-Aldrich ; for 5 min at 4C. Proper lysis of the cell membrane was monitored by dark field microscopy. Nuclei were collected by centrifugation at 300 g for 10 min at 4C, resuspended in 500 l of nuclear freezing buffer containing 50 mM Tris-HCl pH 8.3 ; , 40% glycerol, 5 mM MgCl2, and 0.1 mM EDTA, and stored at 80C until used for nuclear run-on analysis. Nuclear run-on and RNA isolation were performed in the presence of biotin-16-UTP Roche ; . Dynabeads M-280 Invitrogen Life Technologies ; were used to capture and purify the biotin-labeled RNA molecules that were produced during the nuclear run-on period. Beads attached to RNA were washed twice with 2 SSC plus 15% formamide, once with 2 SSC, and resuspended in 30 l diethyl pyrocarbonatetreated H2O before the preparation of random hexamer-primed cDNA. Semiquantitative real-time PCR was preformed from serially diluted cDNA samples for the levels of actin and mature IgE transcripts. The OD values obtained for IgE were normalized to -actin. Samples that did not contain biotin-16-UTP served as negative controls and were found to be negative for the presence of -actin and mature IgE transcripts and cinacalcet. 2 3 . For all three solutions there are no significant lower bounds on | m because mutual compensations between the terms contributing to | m corresponding to the exchange of different virtual massive Majorana neutrinos, are possible. In the case of the inverted hierarchy mass spectrum, the dominant contribution to | m due to the two heavier Majorana neutrinos 2, 3 and | m | determined by m2 , sin2 2 , atm the CP-violating phase 31 - 21 and by |Ue1 |2 , constrained by the CHOOZ data as: | m | |Ue1 |2 ; 1 - sin2 2 sin2 31 - 21 ; 2. atm 3. The data on ovarian measurements a series of measurements of ovaries different ages. Only determinations are given in Table I ; each row represents from 6-day-old females treated at the two of right ovaries are included, since data for and cisplatin. Eq.VII in that it is applicable for all data sets. Eqs. IV and V gave the lowest PBA values 1.96% and 0% ; while the corresponding values of Eqs. II and III were 15.69 and 16.67% which were slightly less than those of Eq. I and VI. CONCLUSION Solubility prediction based upon the minimum number of experiments is valuable from practical point of view, especially in the preformulation studies of a new drug that only small amount of the drug is available and in the processes involving temperature changes. The solubility predictions at different temperatures using the solubility at 25 C are proposed employing Eqs. VI and VII, which have a single constant term and their accuracy and prediction capability were much superior to those of pure predictive models Eqs. IV and V ; but overall comparable to those of two and three parameter models Eqs. I III ; requiring two and three experimental solubilities.

Significantly benefited a sector or community in the Philippines, or advanced the cause of overseas Filipino communities. The Banaag Award was given "in recognition of the remarkable achievements in showcasing the diversity of Filipino heritage through world class dance and musical performance and bringing the Philippines closer to overseas Filipinos and the rest of the world." Since its birth, the dance company has received many accolades. Its world class shows have earned the company performances to many prominent events, such as the Olympic Games in Mexico City and Montreal, the Commonwealth Games in Edmonton, the Montreal Expo as well as the opening festivities of both Ontario Place and Skydome - now the Rogers Centre. Fiesta has also become an Ambassador for both Canada and the Philippines. Through it international travels, the troupe has enchanted audiences with their exceptional traditional Philippine folk dance repertoire. In 1991, "Fiesta" burst onto the international scene as one of Canada's representatives at a folklore festival in Portugal. The success of this first European appearance led to a flurry of international invitations, many of which Fiesta accepted. Today, this leading Filipino dance troupe in North America has performed on stages in France, Italy, Greece, Austria, Spain, USA and the Philippines. Behind the grandeur and the accolades of a successful performance lies a tireless organization. Through their efforts, the members were taught the various forms of Philippine dance that have been influenced by the archipelago's unique geography and colorful history. The layers that comprise the Filipino people are told through the mountain tribes of Banaue Rice Terraces, the aristocracy during Spanish colonization, the story of courtship between a young Muslim prince and princess, the dances of the various indigenous tribes and the dances of the lively countryside. George and Estring's inspired research has taken them across the islands and it has allowed both the dance troupe's performing artists and audience members to learn more of the Philippines and its people through music, song and dance. The repertoire of the Fiesta Filipina Dance Troupe is traditionally divided into five main suites that highlight the cultural diversity of the Filipinos: Cordillera - These dances are based on the culture and traditions of the mountain tribes of the Cordillera region in the island Luzon. Known collectively as Igorots, they are the builders of one of the Forgotten Wonders of the World, the Banaue Rice Terraces. Their dances are performed to appease their gods, to ward off bad spirits, to ensure bountiful harvests and peace, and to celebrate successful battles in war. Spanish - These dances depict the cultural influences of the Spanish conquistadors on the Filipino people. These dances merge the Spanish jota, the flamenco, and period costumes of the 18th and 19th centuries with the Filipino culture in the form of bamboo castanets, bamboo canes and the "abanico, " or Asian fan. Morolandia - These dances are portrayed by the Muslim Filipinos that reside in the southern island of Mindanao and in the Sulu Archipelago. The dances are known for their Arabian and Indo-Malaysian influences. Tribal - These dances are those performed by the various indigenous tribes and cultural minorities that reside throughout the Philippine archipelago and cladribine. The patients monitored consisted of 68 males and 86 females, ranging in age from six weeks to 86 years Table 1 ; . Most of the.
Intact ADLs can be as simple as still being able to dress, wash Or, intact IADLs e.g. driving car, managing housework, catching public transport But, are subtle impairments included? Eg ability to weigh up competing investment portfolios and make decisions about finance and clofarabine.

The high-resolution mode 6.5 mm f ull width at half maximum ; , over a 90 min period. Baboons were positioned so that the lowest plane was located approximately 5 cm below the canthomeatal line. Plasma radioactivity was corrected for decay and metabolites. In all cases, 30 arterial samples PET study for radioassay and protein binding ; were obtained over 90 min. To correct the input f unction for unmetabolized [ 11C]W I N35, 428, arterial samples were also obtained at 10, 20, 30, and 75 min after the injection of the radiotracer for analysis by means of high performance liquid chromatography HPLC ; . PET images were reconstructed from the raw data with a standard filtered back-projection algorithm and a Hann filter 6.0 mm ; . Images were corrected for attenuation and decay. Regions of interest were placed manually over the striatum and the cerebellum, and time-activity curves were generated. Postmortem monoamine and metabolite level determinations. Levels of monoamines and their major metabolites were determined by means of HPLC coupled with electrochemical detection HPLC -EC ; , as described previously Ricaurte et al., 1992 ; . In vitro [ 3H]W IN-35, 428 binding studies. [ 3H]W I N-35, 428-labeled DATs were measured using the method of Madras and colleagues 1989 ; , with minor modification. Briefly, frozen striatal tissue was weighed, homogenized in 20 vol w v ; of 0.32 M sucrose phosphate buffer, pH 7.4, at 0 4C, and centrif uged at 45, 000 g for 15 min at 0 4C. The supernatant was discarded, and the pellet was resuspended in 20 vol of sucrose PO4 buffer and then centrif uged once again at 45, 000 g for 15 min at 0 4C. The resulting pellet was suspended in buffer for a final tissue concentration of 10 mg ml wet weight. [ 3H]W I N-35, 428 was used at a predetermined saturating concentration of 30 nM. Cocaine, at a final concentration of 30 M, was used to displace specific [ 3H]W I N-35, 428 binding and estimate nonspecific binding. T ubes were incubated in sextuplicate for 60 min, at 0 4C. Membranes were harvested by filtration through Whatman GFB filters soaked in 0.05% polyethyleneimine PEI ; . Filters were washed three times using ice-cold sucrose PO4 buffer. Radioactivity was measured with a Packard-1500 Tricarb Liquid Scintillation Analyzer. Specific [ 3H]W I N35, 428 binding was calculated by subtracting the average value of the six tubes containing excess cocaine from the average of the six tubes without cocaine. Specific [ 3H]W I N35, 428 binding was expressed in dpm mg original wet weight tissue. Nonspecific binding represented 710% of the total binding. In vitro [ 3H]dihydrotetrabenazine TBNZ ; binding studies. [ 3H]DTBZ binding, used to label type 2 vesicular monoamine transporter V M AT ; sites Naudon et al., 1994; Vander Borght et al., 1996 ; , was measured using the method of Wilson et al. 1996b ; , with minor modifications. Briefly, tissue samples were homogenized for 15 sec in 20 vol w v ; of sodium phosphate buffer 25 mM, pH 7.7 ; and then centrif uged in a Sorvall RC2B at 45, 000 g for 15 min at 0 4C. The resulting pellet was resuspended in 20 vol w v ; of sodium phosphate buffer, homogenized again for 15 sec, and recentrif uged at 45, 000 g for 15 min at 0 4C. The supernatant was discarded, and the resulting pellet was resuspended in buffer at a final concentration of 10 mg of original wet weight tissue per milliliter. Membrane preparations were incubated with a predetermined saturating concentration of [ 3H]DTBZ 15 nM ; in sodium phosphate buffer, pH 7.7, for 90 min at 30C in a shaking water bath. Each sample was run in sextuplicate, such that six tubes were used to define total binding and six tubes were used to determine nonspecific binding. Nonspecific binding was determined in the presence of 1 M tetrabenazine and represented 8 10% of total binding. The incubation was terminated by rapid filtration, using a 48-well cell harvester Brandell, Gaithersburg, MD ; and Whatman GFB filters soaked with 0.05% PEI. Filters were washed three times with 10 ml sodium phosphate buffer, and residual radioactivity was measured using a Packard-1500 Tricarb Liquid Scintillation Analyzer. Specific binding, calculated by subtracting nonspecific binding from total binding, was expressed as dpm mg original wet weight tissue. In vitro [ 3H]paroxetine binding studies. [ 3H]paroxetine-labeled 5-HTT were measured using the method of Habert et al. 1985 ; , with the previously described minor modifications Ricaurte et al., 1992 ; . In vitro [ 3H]nisoxetine binding studies. [ 3H]nisoxetine-labeled norepinephrine transporters N ETs ; in tissue homogenates were measured using the method of Tejani-Butt 1992 ; . Autoradiographic studies. Autoradiograms with [ 3H]W I N-35, 428 [ 3H]-2 -carbomethoxy-3 - 4-fluorophenyl ; -tropane ; were prepared according to the method of Kaufman et al. 1991 ; . [ 3H]W I N-35, 428 was used at a concentration of 3 nM measure total binding and along with 30 M cocaine to determine nonspecific binding. Autoradiograms with [ 3H]mazindol were prepared using the method of Javitch et al. 1985 and clove. Go to the emergency room if: the injured area is bent, twisted, or a bone is poking through the skin!

Exchanges of scholars and professors. The University of Los Andes has a Department of Afro-Asian Studies with some renowned Indologists. There have been relatively few exchanges of students, largely because of the language difficulties. ITEC In the past, utilization of ITEC slots has been rather slow and sporadic, mainly on account of language difficulties faced by Venezuelans. However, the five slots allocated by the Ministry of External Affairs for the year 2004-5 have been fully utilized. Indian Community There are about 50 Indian families in Venezuela, of which about 35 are in Caracas. These comprise mostly businessmen, professionals and professors. At the initiative of the Embassy, the Indian Association of Venezuela, which was dormant since 1997, was revived in 2003. The Association has since been organizing several activities on Diwali, Holi, etc.

The year 2002 is likely to be remembered as the annus mirabilis of solar neutrino physics. On April 20, direct and highly significant evidence for e flavor change into active states was announced by the Sudbury Neutrino Observatory SNO ; experiment [1], crowning a four-decade long [2] series of beautiful observations [3, 4, 5, 6, of the solar e flux deficit [12, 13]. On October 8, the role of solar neutrino physics in shaping modern science was recognized through the Nobel Prize jointly awarded to Raymond Davis, Jr., and Masatoshi Koshiba, for their pioneering contributions to the detection of cosmic neutrinos [14]. Finally, on December 6, clear "terrestrial" evidence for the oscillation solution to the solar neutrino deficit was reported by the Kamioka Liquid scintillator AntiNeutrino Detector KamLAND ; , through the observation of long-baseline reactor e disappearance [15]. The seminal idea of studying lepton physics by detecting solar [16, 17] and reactor [16, 17, 18] neutrinos keeps thus bearing fruits after more than 50 years. The KamLAND observation of e disappearance [15] confirms the current interpretation of solar neutrino data [9, 11, 19, 20, in terms of e , oscillations induced by neutrino mass and mixing [22, 23], and restricts the corresponding parameter space m2 , 12 ; within the so-called large mixing angle LMA ; region. In this region, globally favored by solar neutrino data [24], matter effects [25, 26] in adiabatic regime [25, 27] are expected to dominate the dynamics of flavor transitions in the Sun see, e.g., [28] ; . The KamLAND spectral data appear to exclude some significant portions of the LMA solution [15], where the predicted spectrum distortions [21, 29, 30, 31, would be in conflict with observations [15]. In this paper we analyze the first KamLAND spectral data [15] and combine them with current solar neutrino data [1, 3, 5, 6, assuming two- and three-flavor oscillations of active neutrinos [21], in order to determine the surviving sub-regions of the LMA solution. In the analysis we include the CHOOZ reactor data [38] and, in the 3 case, also the relevant constraints on the larger mass gap m2 m2 , coming from the Super-Kamiokande SK ; atmospheric [39] and KEK-to-Kamioka K2K ; accelerator [40, 41] neutrino experiments, according to the approach developed in [21].1 In the 2 case, we find that the inclusion of the KamLAND spectrum basically splits the LMA solution into two sub-regions at "small" and "large" m2 , which we call LMA-I and LMA-II, respectively the LMA-I solution being preferred by the data ; . Such regions are only slightly modified in the presence of 3 mixing, namely, for nonzero values of the mixing angle 13 . We also present updated bounds in the 3 parameter space m2 , 12 , 13 ; The structure of this paper is as follows. In Sec. II we describe our analysis of the KamLAND data. In Sec. III we combine such measurements with solar and CHOOZ data, assuming 2 mixing. In Sec. IV we extend the analysis to 3 mixing, including the SK + K2K terrestrial neutrino constraints. We draw our conclusions in Sec. V.
Articles in medical journals, healthcare publications, and even national media such as ABC News, The Boston Globe, The Los Angeles Times, The New York Times, Business Week, and The Wall Street Journal have featured Intermountain as an example of what's right in American healthcare. "We talk about `Healing for Life' and our passion for providing care that is excellent in every respect, " said Dr. James. "I think the physicians, nurses, and other. Struggle to accept that people with life-limiting illness, especially older people, continue to be sexual beings. People facing life-limiting illness may appreciate the opportunity to discuss issues of sexuality and intimacy with a trusted health professional. Practical strategies to assist health professionals to communicate effectively about sexuality and intimacy include creating a conducive atmosphere, initiating the topic, using open-ended questions and a non-judgemental approach, and avoiding medical jargon. 10 MISCELLANEOUS Pelvic ring disruptions in emergency radiology. Stambaugh LE, Blackmore CC. Eur J Radiol 2003; 48: 71-87. Pelvic ring disruptions are a result of high-energy trauma and are a significant cause of morbidity and mortality in major trauma patients. The initial pelvic radiograph, in combination with computed tomographic imaging in selected patients, provides a quick and accurate method of diagnosing pelvic fractures. Pelvic fracture classification schemes have evolved over the past few decades, and include description of the mechanism of injury and assessment of pelvic stability. Understanding these classifications is important in developing an approach to interpretation of pelvic imaging and prediction of associated injuries. Armed with these tools, the emergency radiologist can detect pelvic fractures early and guide subsequent imaging and therapy. Clinical and economic choices in anaesthesia for day surgery. Lake AP, Khater M. Anaesthesia 2003; 58: 1031-2. Consent and anaesthetic risk. Jenkins K, Baker AB. Anaesthesia 2003; 58: 962-84. The incidences of mortality and morbidity associated with anaesthesia were reviewed. Most of the published incidences for common complications of anaesthesia vary considerably. Where possible, a realistic estimate of the incidence of each morbidity has been made, based on the best available data. Perception of risk and communication o anaesthetic risk to patients are discussed. The incidences of anaesthetic complications f are compared with the relative risks of everyday events, using a community cluster logarithmic scale, in order to place the risks in perspective when compared with other complications and with the inherent risks of surgery. Documentation of these risks and discussion with patients should allow them to be better informed of the relative risks of anaesthetic complications. Depending on specific comorbidities and the severity of operation, these risks associated with anaesthesia may increase for any one individual. Tragic History of the VII International Congress of Genetics. Soyfer VN. Genetics 2003; 165: 1-9. Antioxidant effects of tea: evidence from human clinical trials. Rietveld A, Wiseman S. J Nutr 2003; 133: 3285S-92S. Tea remains the most consumed drink in the world after water, well ahead of coffee, beer, wine and carbonated soft drinks. An accumulated number of population studies suggests that consumption of green and black tea beverages may bring positive health effects 1 ; . One hypothesis explaining such effects is that the high levels of flavonoids in tea can protect cells and tissues from oxidative damage by scavenging oxygen-free radicals. Chemically, the flavonoids found in green and black tea are very effective radical scavengers. The tea flavonoids may therefore be active as antioxidants in the digestive tract or in other tissues after uptake. A substantial number of human intervention studies with green and black tea demonstrates a significant increase in plasma antioxidant capacity in humans approximately 1 h after consumption of moderate amounts of tea 1-6 cups d ; . There are initial indications that the enhanced blood antioxidant potential leads to reduced oxidative damage to macromolecules such as DNA and lipids. However, the measurement of oxidative damage through biomarkers needs to be further established. In conclusion, tea flavonoids are potent antioxidants that are absorbed from the gut after consumption. Tea consumption consistently leads to a significant increase in the antioxidant capacity of the blood. Beneficial effects of increased antioxidant capacity in the body may be the reduction of oxidative damage to important biomolecules. The scientific support is strongest for the protection of DNA from oxidative damage after black or green tea consumption. However, the quality of the studies now available is insufficient to draw firm conclusions. Therefore, further evidence from human intervention studies is required and cilium. Some reluctance to transferring the responsibility of chronic care from a physician to another health care professionals. Although the movement to deregulate more prescription drugs to OTC status may result in an economic loss for pharmacists, owing to fewer prescriptions, it also creates a potential opportunity for gain by significantly broadening the scope of practice from primarily a dispensing role to a clinical, evaluative and consultative role, for which professional services could be reimbursed. This also raises the question of whether pharmacists should be, and need to be, granted prescribing authority, as is being pursued by the Alberta College of Pharmacists, or whether prescription drugs should merely be deregulated to nonprescription, behind-the-counter no public access ; status e.g., emergency contraception ; , for which an interaction with a pharmacist is required. Although some previous nonscientific evaluations of pharmacists' involvement or intervention in the provision of nonprescription drugs have not been favourable, 1618 the recently published experience of pharmacists prescribing emergency contraception1 provides evidence of the feasibility and effectiveness of such a program with appropriate planning, infrastructure and remuneration. Not requiring a physician visit on a continuing basis also has other economic implications. Although the shifting of some of the responsibilities for the care of chronic diseases from physicians to other health care providers may be a logical step in maintaining an acceptable level of health care given the current physician shortage, it will also probably increase the overall complexity of the conditions physicians will see.19 More complex cases require more time, which will necessitate a concomitant change in how professional services are remunerated. In fact, the provision of professional services by other health care professionals will require that the current fee schedules for all health care providers be reviewed and revised, as has already been done for pharmacists in some jurisdictions. The movement to deregulate more prescription drugs to OTC status for the management of chronic diseases may be seen as a logical step toward meeting the demands of today's health care environment and promoting the rights and health of patients. However, the overall objective of such a change must be improved health care provision and patient outcomes. Given the sweeping changes that have already occurred and those being proposed in other jurisdictions in line with the Romanow report20 as well as the need for further budget constraints, similar changes for Canada will be an inevitable consideration. Although in the short term physicians will probably maintain a primary role in choosing drug therapies regardless of the status of the drug, the roles and responsibilities of all health care providers will probably undergo further changes over the long term. The changes seen in making emergency contraception available OTC are merely the tip of the iceberg.21 Now is the time to. WARES: Bags, namely, sports bags, athletic bags, beach bags, duffel bags, gym bags, overnight bags, and shoulder bags; shorts, shirts, socks, pants, jackets, vests, hats, bras, gloves, headbands, underwear, and swimsuits. Used in CANADA since as early as December 2003 on wares. MARCHANDISES: Sacs, nommment sacs de sport, sacs d'athltisme, sacs de plage, sacs polochon, sacs de sport, sacs de voyage et sacs bandoulire; shorts, chemises, chaussettes, pantalons, vestes, gilets, chapeaux, soutiens-gorge, gants, bandeaux, sous-vtements et maillots de bain. 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Confirmed that this change was not due to an error accumulated during PCR by sequencing the RT-PCR products directly. Stable expression of Oatp14 cDNA in HEK293 cells. The Oatp14-cDNA was subcloned into the pcDNA3.1 + ; Invitrogen, CA, USA ; , and introduced into HEK293 cells by lipofection with FuGENE6 Roche Diagnostics, Basel, Switzerland ; according to the manufacturer's protocol and were selected by culturing them in the presence of G418 sulfate 800 g ml ; BRL, Gaithersburg, MD ; . HEK293 cells were grown in.