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Ced and changed during the cell cycle. One of the reasons for the observed phenomenon may be related to the properties of the cell wall and the morphogenetic processes of yeast on the S-phase of the cell cycle which include: localisation of secretion of other materials to the surface of the bud; localisation of new cell-wall growth to the tip of the bud during much of the period of the bud growth [15]. Several studies have shown that the yeast cell wall is not a static shield but a highly dynamic structure that can change according to the physiological needs of the cell. During a cell cycle, the cell wall has to be remodelled to be more plastic in the point of bud emergence where the growth takes place. The rapid growth of buds suggests that the events ruling the development of such an apparently rigid structure may allow a certain flexibility, at least in growing areas, without altering the protective function of the cell wall. In this regard, it has been revealed that the cell wall of S. cerevisiae exhibits variations in porosity during growth and cell division. Maximum porosity is observed during the bud growth where the wall is in a more plastic, expanded state as compared with stationary phase cells [16, 17]. Furthermore, yeast cells subjected to a heat shock may become weaker, and this leads to plasma membrane stretching. All the mentioned and other concomitant events facilitate the penetration of the plasmid DNA by increasing the permeability of the cell envelope to large molecules, including exogenous DNA. A variety of methodologies exist for the transport of exogenous DNA into the cell interior, and usually it is achieved in two ways: natural transport and that induced with various specific treatment. A minor subpopulation never exceeding 20% ; of bacterial cells in a given culture develops competence under specific growth and developmental process. The best characterised naturally transformable bacteria are the Gram-negative species Haemophillus influenza [18] and the Gram-positive Streptococcus pneumoniae and Bac. subtilis [19, 20]. However, it should be noted that the competence of prokaryotic cells appears as a physiological state during the cell growth. The problem of the possibilities of the phenomenon of competence in yeast cells is not discussed on the same level as with bacterial cells. It has been noted that cells growing exponentially are competent to take up DNA [6, 21, 22], whereas the results presented in this study indicate that yeast cells in S-phase are more sensitive to DNA and the efficiency of yeast transformation seems to be comparable with the efficiency of genetic transformation of Bac. subtilis in the state of competence [23]. Direct insertion and integration of the functionally ac.
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